| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
18/10/2010 |
|
Data da última atualização: |
19/10/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
ANDRADE, E. C.; SANTOS, K. C.; MEISSNER, P. E. |
|
Afiliação: |
EDUARDO CHUMBINHO DE ANDRADE, CNPMF; Keila Cidreira Santos, FAMAM; PAULO ERNESTO MEISSNER FILHO, CNPMF. |
|
Título: |
Development of degenerated primers to detect different viruses associated with mealybug wilt of pineapple. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. |
|
Idioma: |
Inglês |
|
Notas: |
Disponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011.
Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. |
|
Conteúdo: |
Pineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing. MenosPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results s... Mostrar Tudo |
|
Palavras-Chave: |
Pineapple; Plant disease. |
|
Thesagro: |
Abacaxi; Doença de Planta. |
|
Thesaurus Nal: |
Ananas comosus var. comosus. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02561nam a2200205 a 4500
001 1864522
005 2023-10-19
008 2010 bl uuuu u00u1 u #d
100 1 $aANDRADE, E. C.
245 $aDevelopment of degenerated primers to detect different viruses associated with mealybug wilt of pineapple.$h[electronic resource]
260 $aPineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S.$c2010
500 $aDisponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011. Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010.
520 $aPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing.
650 $aAnanas comosus var. comosus
650 $aAbacaxi
650 $aDoença de Planta
653 $aPineapple
653 $aPlant disease
700 1 $aSANTOS, K. C.
700 1 $aMEISSNER, P. E.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 112 | |
| 11. |  | GOMES, V. B.; SANTOS, K. C. F. dos; SOUZA, A. da S.; SANTOS, V. da S. Conservação in vitro de variedades do banco de germoplasma de mandioca (Manihot esculenta Crantz). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 13. | | CARVALHO, C. dos S. B.; SANTOS, K. C. L. dos; ALMEIDA, F. N.; ARBEX, W. A. Desenvolvimento e implementação de um sistema de backup para ambiente de computação científica com infraestrutura de baixo custo. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 18. | | GOMES, S. E. V.; SANTOS, K. C.; OLIVEIRA, G. M. de; ARAÚJO, M. N.; DANTAS, B. F. Sementes de Myracrodruon urundeuva podem ser armazenadas por até dois anos em ambiente seco. Informativo Abrates, v. 28, n. 1, p. 102-106, 2018. Edição Especial. 6 Workshop de Sementes e Mudas da Caatinga, Juazeiro-BA/Petrolina-PE, jul./ago. 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 19. | | SANTOS, K. C. L. DOS; ARBEX, W. A.; NASCIMENTO, E. DE O.; MARTINS, M. F.; SILVA, M. V. G. B. SireDNA: um sistema de informação para armazenamento e recuperação de dados do banco de DNA de bovinos de leite. In: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Unir talentos para ultrapassar fronteiras. Fortaleza: Embrapa Agroindústria Tropical, 2014. p. 9.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 20. | | SOUZA, F. V. D.; ANDRADE, E. C. de; JUNGHANS, D. T.; CARVALHO, H. L.; SANTOS, K. C. dos. Cultivo de meristemas apicais de plantas in vitro para limpeza viral em abacaxi. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. 1 CD-ROM. pdf 703| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| Registros recuperados : 112 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
